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KMID : 0043320010240030207
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Archives of Pharmacal Research
2001 Volume.24 No. 3 p.207 ~ p.210
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High Perormance Liquid Chromatogrphic Analysis of a New Proton Pump Inhibitor KR60436 and Its Active Metabolite 0-Demethyl-KR60436 in Rat Plasma Samples Using Column-Switching
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Lee HM
Lee HY/Choi JK/Lee HS
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Abstract
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A fully automated high performance liquid chromatography with column-switching was deyeloped for the simultaneous determination of KR60436, a new reyersible proton pump iuhibitor, and its active. Metabolite 0-demethyl-KR604367 from rat plasma samples. Plasma sample (50mcl) was directly introduced onto a Capcell Pak MF Ph-1 column (10*4 mm I.D.) where primary Separatior was occurred to remoye proteins and concentrate target substances using acetonitrile-potassium phosphate (Ph 7, 0.1M) (2:8, v/v). The drug molecules eluded from MF Ph-1 column were focused in an intermediate column (10*2 mm I.D.) by the valve switching step. The substances enriched in intermediate column were eluted and separated on a Vydac 218MR53 column (250*3.2mm I.D) using acetonitrilepotassium phosphate (pH 7,0.02M) (47:53, v/v) at a flow rate of 0.5 ml/min when the va1ve status was switched back to A position. The method showed excellent sensitivity(detection limit of 2ng/ml) with small volume of samples (50ml), good precision and Accuracy and speed (total ana1ysis time 24 min) without any loss in chromatofraphic efficience. The response was linear (r2 ≥ 0.999) over the concentration range of 5-500 ng/ml.
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